Composite

Part:BBa_K4425009:Design

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


pNEG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 93
    Illegal NheI site found at 2136
    Illegal PstI site found at 1690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 93
    Illegal BglII site found at 82
    Illegal BamHI site found at 1871
    Illegal XhoI site found at 424
    Illegal XhoI site found at 1501
    Illegal XhoI site found at 2458
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 93
    Illegal XbaI site found at 85
    Illegal PstI site found at 1690
    Illegal NgoMIV site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2014
    Illegal BsaI.rc site found at 2254
    Illegal SapI site found at 1326
    Illegal SapI site found at 2394
    Illegal SapI.rc site found at 1716
    Illegal SapI.rc site found at 2304


Design Notes

a


Source

a

References